منابع مشابه
607397: Separation of DNA by Capillary Electrophoresis
(under the direction of Prof. B. L. Karger), and a M.S. degree from the Free University, Am-sterdam. He has been working in the analytical instrumentation industry for over 10 years, has authored approximately 50 publications in the field of separation science, and taught training courses in capillary electrophoresis. Before starting his consulting business, Palomar Analytical Services in Redwo...
متن کاملIntroducing a New and Simple Protocol for Capillary Electrophoresis of Cell Free Fetal Double Stranded DNA
Isolation of cell free fetal DNA (cffDNA) from maternal serum usually leads to very low concentrations of DNA impeding further resolving through conventional methods of electrophoresis. Although several protocols have been described for capillary electrophoresis (CE) of double stranded DNA, they usually need using special polymers or coated capillaries which degrade over time. Herein, we propos...
متن کاملDNA sequencing by capillary electrophoresis (review).
DNA sequencing by capillary electrophoresis has been reviewed with an emphasis on progress during the last four years. The effects of sample purification, composition of sieving matrices, electric field strength, temperature, wall coating and DNA labeling on the DNA sequencing performance are discussed. Multicapillary array instrumentation is compared with one-capillary systems. Integrated syst...
متن کاملHigh speed DNA sequencing by capillary electrophoresis.
A major challenge of the Human Genome Initiative is the development of a rapid, accurate, and efficient DNA sequencing technology. A major limitation of current technology is the relatively long time required to perform the gel electrophoretic separations of DNA fragments produced in the sequencing reactions. We demonstrate here that it is possible to increase the speed of sequence analysis by ...
متن کاملMonitoring viral DNA release with capillary electrophoresis.
Viral DNA injection into host cells is one of the primary mechanisms of viral propagation. Drug development that targets viral propagation requires fast and sensitive methods for monitoring the release of viral DNA in vitro. Here we demonstrate the use of capillary electrophoresis (CE) for monitoring DNA release from virus particles. As a model for this study, we used T5 bacteriophages that inf...
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ژورنال
عنوان ژورنال: Current Protocols in Molecular Biology
سال: 2004
ISSN: 1934-3639,1934-3647
DOI: 10.1002/0471142727.mb0208s68